EGLN2 Antibody

• Background:

  The hypoxia inducible factor (HIF) is a transcriptional complex which is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. EGLN2 encodes an enzyme responsible for this posttranslational modification. Alternative splicing of EGLN2 results in three transcript variants encoding different isoforms.

• NCBI Gene ID:

  112398

• Swiss Prot:

  Q96KS0

• Accession Number:

  NP_444274

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EGLN2.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription, Cancer, Signal Transduction, Immunology

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. 1309 - Human Placenta Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store EGLN2 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  44 kDa

• Applications Notes:

  EGLN2 antibody can be used for detection of EGLN2 by ELISA at 1:1562500. EGLN2 antibody can be used for detection of EGLN2 by western blot at 0.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  EGLN2

• NCBI Official Name:

  Egl-9 family hypoxia-inducible factor 2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Appelhoff, R.J., et al., (2004) J. Biol. Chem. 279 (37), 38458-38465.

• Other Product Names:

  EGLN2, EIT6, PHD1, HPH-1, HPH-3, HIFPH1, HIF-PH1

• Tested Applications:

  ELISA, WB

• Protein ID:

  16604260

• Physical Properties:

  Liquid