EGLN1 Antibody

• Background:

  The protein encoded by this gene catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. This protein functions as a cellular oxygen sensor, and under normal oxygen concentration, modification by prolyl hydroxylation is a key regulatory event that targets HIF subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex. Mutations in this gene are associated with erythrocytosis familial type 3 (ECYT3) .

• NCBI Gene ID:

  54583

• Swiss Prot:

  Q9GZT9

• Accession Number:

  Q9GZT9

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This EGLN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 396-425 amino acids from the C-terminal region of human EGLN1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Obesity

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  46 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  EGLN1

• NCBI Official Name:

  Egl nine homolog 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Aggarwal, S., et al. Proc. Natl. Acad. Sci. U.S.A. 107 (44) :18961-18966 (2010)

• Background Reference 02:

  Vogel, S., et al. J. Biol. Chem. 285 (44) :33756-33763 (2010)

• Background Reference 03:

  Simonson, T.S., et al. Science 329 (5987) :72-75 (2010)

• Background Reference 04:

  Thoms, B.L., et al. J. Biol. Chem. 285 (27) :20472-20480 (2010)

• Other Product Names:

  HGNC:1232

• Tested Applications:

  WB

• Protein ID:

  32129514

• Physical Properties:

  Liquid