POLE3 Antibody

• Background:

  POLE3 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.

• NCBI Gene ID:

  54107

• Swiss Prot:

  Q9NRF9

• Accession Number:

  NP_059139

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat, Dog

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human POLE3.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cell Cycle

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. 1224 - Daudi Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store POLE3 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  17 kDa

• Applications Notes:

  POLE3 antibody can be used for detection of POLE3 by ELISA at 1:312500. POLE3 antibody can be used for detection of POLE3 by western blot at 2.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  POLE3

• NCBI Official Name:

  Polymerase (DNA directed), epsilon 3, accessory subunit

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Bolognese, F., et al., (2000) Nucleic Acids Res. 28 (19), 3830-3838.

• Other Product Names:

  POLE3, p17, YBL1, CHRAC17, CHARAC17

• Tested Applications:

  ELISA, WB

• Protein ID:

  224586804

• Physical Properties:

  Liquid