PTK2B Antibody

• Background :

  PTK2B encodes a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies.

• NCBI Gene ID :

  2185

• Swiss Prot :

  Q14289

• Accession Number :

  NP_004094

• Host :

  Rabbit

• Reactivity :

  Human, Mouse, Rat, Dog

• Immunogen :

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PTK2B.

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Cancer, Signal Transduction, Apoptosis

• Purification :

  Antibody is purified by protein A chromatography method.

• Positive Control :

  Cat. No. 1207 - Raji Cell Lysate

• Concentration :

  Batch dependent

• Buffer :

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store PTK2B antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight :

  116 kDa

• Applications Notes :

  PTK2B antibody can be used for detection of PTK2B by ELISA at 1:312500. PTK2B antibody can be used for detection of PTK2B by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol :

  PTK2B

• NCBI Official Name :

  Protein tyrosine kinase 2 beta

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Ling, S., et al., (2004) Am. J. Cell Physiol. 287 (3), C698-C706.

• Other Product Names :

  PTK2B, PKB, PTK, CAKB, FAK2, PYK2, CADTK, FADK2, RAFTK

• Tested Applications :

  ELISA, WB

• Protein ID :

  27886584

• Physical Properties :

  Liquid