HCLS1 Antibody

• Background:

  HS1 which is hematopoietic lineage cell-specific protein 1, is a substrate of protein tyrosine kinases in lymphocytes, it binds to F-actin, and promotes Arp2/3 complex-mediated actin polymerization. However, the mechanism for the interaction between HS1 and F-actin has not yet been fully characterized. HS1 contains 3.5 tandem repeats, a coiled-coil region, and an SH3 domain at the C terminus. Unlike cortactin, which is closely related to HS1 and requires absolutely the repeat domain for F-actin binding, an HS1 mutant with deletion of the repeat domain maintains a significant F-actin binding activity. Deletion of the coiled-coil region abolished the ability of HS1 to bind to actin filaments and to activate the Arp2/3 complex for actin nucleation and actin branching.

• NCBI Gene ID:

  3059

• Swiss Prot:

  P14317

• Accession Number:

  NP_005326

• Host:

  Rabbit

• Reactivity:

  Human, Rat

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HCLS1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription, Signal Transduction, Immunology

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. 1205 - Jurkat Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store HCLS1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  54 kDa

• Applications Notes:

  HCLS1 antibody can be used for detection of HCLS1 by ELISA at 1:2500. HCLS1 antibody can be used for detection of HCLS1 by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  HCLS1

• NCBI Official Name:

  Hematopoietic cell-specific Lyn substrate 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Ruzzene, M., et al., (2002) Biochem. J. 364 (Pt 1), 41-47.

• Other Product Names:

  HCLS1, HS1, CTTNL

• Tested Applications:

  ELISA, WB

• Protein ID:

  167234422

• Physical Properties:

  Liquid