NCOR2 Antibody

• Background:

  NCOR2 forms a large corepressor complex that contains SIN3A/B and histone deacetylases HDAC1 and HDAC2. This complex associates with the thyroid (TR) and the retinoid acid receptors (RAR) in the absence of ligand, and may stabilize their interaction with TFIIB. NCOR2 mediates the transcriptional repression activity of some nuclear receptors by promoting chromatin condensation, thus preventing access of the basal transcription.Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests.

• NCBI Gene ID:

  9612

• Swiss Prot:

  Q9Y618

• Accession Number:

  NP_006303

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NCOR2.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription, Cancer

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. 1201 - HeLa Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store NCOR2 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  275 kDa

• Applications Notes:

  NCOR2 antibody can be used for detection of NCOR2 by ELISA at 1:62500. NCOR2 antibody can be used for detection of NCOR2 by western blot at 2.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  NCOR2

• NCBI Official Name:

  Nuclear receptor corepressor 2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Keeton, E.K. (2005) Mol. Endocrinol. 19 (6), 1543-1554.

• Other Product Names:

  NCOR2, SMRT, TRAC, CTG26, SMRTE, TRAC1, N-CoR2, TNRC14, TRAC-1, SMAP270, SMRTE-tau

• Tested Applications:

  ELISA, WB

• Protein ID:

  331284178

• Physical Properties:

  Liquid