TAF3 Antibody

• Background:

  Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. TAF3 is required in complex with TBPL2 for the differentiation of myoblasts into myocytes. The complex replaces TFIID at specific promoters at an early stage in the differentiation process.

• NCBI Gene ID:

  83860

• Swiss Prot:

  Q5VWG9

• Accession Number:

  NP_114129

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TAF3.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Positive Control:

  Cat. No. 1211 - HepG2 Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store TAF3 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  103 kDa

• Applications Notes:

  TAF3 antibody can be used for detection of TAF3 by ELISA at 1:12500. TAF3 antibody can be used for detection of TAF3 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  TAF3

• NCBI Official Name:

  TAF3 RNA polymerase II, TATA box binding protein (TBP) -associated factor, 140kDa

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Olsen, J.V., (2006) Cell 127 (3), 635-648.

• Other Product Names:

  TAF3, MGC25063, TAF140, TAFII140, TAFII-140

• Tested Applications:

  ELISA, WB

• Protein ID:

  151301171

• Physical Properties:

  Liquid