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TAF6 Antibody

CAT: 0223-57-846Size: 400 µLDry Ice: NoHazardous: No
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CAT#:0223-57-846Size:400 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the smaller subunits of TFIID that binds weakly to TBP but strongly to TAF1, the largest subunit of TFIID. Alternative splicing results in multiple transcript variants.
NCBI Gene ID
6878
Swiss Prot
P49848
Accession Number
P49848
Host
Rabbit
Reactivity
Human
Immunogen
This TAF6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-53 amino acids from the N-terminal region of human TAF6.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Other
Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Batch dependent
Homology
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Buffer
Supplied in PBS with 0.09% (W/V) sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Calculated Molecular Weight
73 kDa
Fragment
Rabbit Ig
Applications Notes
For WB starting dilution is: 1:1000
Symbol
TAF6
NCBI Official Name
Transcription initiation factor TFIID subunit 6
NCBI Organism
Homo sapiens
Background Reference 01
Theisen, J.W., et al. Mol. Cell. Biol. (2010) In press :
Background Reference 02
Wilhelm, E., et al. BMC Mol. Biol. 11, 10 (2010) :
Background Reference 03
Wilhelm, E., et al. PLoS ONE (7), E2721 (2008) :
Background Reference 04
Frontini, M., et al. Mol. Cell. Biol. 25 (11) :4638-4649 (2005)
Other Product Names
Transcription initiation factor TFIID subunit 6, RNA polymerase II TBP-associated factor subunit E, Transcription initiation factor TFIID 70 kDa subunit, TAF (II) 70, TAFII-70, TAFII70, Transcription initiation factor TFIID 80 kDa subunit, TAF (II) 80, TAFII-80, TAFII80, TAF6, TAF2E, TAFII70
Tested Applications
WB
Protein ID
1729810
Physical Properties
Liquid

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