TAF1 Antibody

CAT: 0223-25-069Size: 100 µLDry Ice: NoHazardous: No
CAT#:0223-25-069Size:100 µL
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Background
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is the basal transcription factor TFIID, which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. TAF1 encodes the largest subunit of TFIID. This subunit binds to core promoter sequences encompassing the transcription start site. It also binds to activators and other transcriptional regulators, and these interactions affect the rate of transcription initiation. This subunit contains two independent protein kinase domains at the N and C-terminals, but also possesses acetyltransferase activity and can act as a ubiquitin-activating/conjugating enzyme. Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is the basal transcription factor TFIID, which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes the largest subunit of TFIID. This subunit binds to core promoter sequences encompassing the transcription start site. It also binds to activators and other transcriptional regulators, and these interactions affect the rate of transcription initiation. This subunit contains two independent protein kinase domains at the N and C-terminals, but also possesses acetyltransferase activity and can act as a ubiquitin-activating/conjugating enzyme. Two transcripts encoding different isoforms have been identified for this gene.
NCBI Gene ID
6872
Swiss Prot
P21675-2
Accession Number
NP_004597
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TAF1.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Transcription, Cell Cycle, Signal Transduction
Purification
Antibody is purified by peptide affinity chromatography method.
Positive Control
Cat. No. XBL-10409 - Fetal Liver Tissue Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store TAF1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Calculated Molecular Weight
215 kDa
Applications Notes
TAF1 antibody can be used for detection of TAF1 by ELISA at 1:312500. TAF1 antibody can be used for detection of TAF1 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
TAF1
NCBI Official Name
TAF1 RNA polymerase II, TATA box binding protein (TBP) -associated factor, 250kDa
NCBI Organism
Homo sapiens
Background Reference 01
Herzfeld, T., (2007) Mamm. Genome 18 (11), 787-795.
Other Product Names
TAF1, BA2R, CCG1, CCGS, DYT3, KAT4, NSCL2, OF, P250, TAF2A, TAFII250, XDP, N-TAF1, DYT3/TAF1, TAFII-250, TAF (II) 250
Tested Applications
ELISA, WB
Protein ID
20357585
Physical Properties
Liquid

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