EIF3E Antibody

• Background:

  EIF3E belongs to the eIF-3 subunit E family.It is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. EIF3E is required for nonsense-mediated mRNA decay (NMD) ; It may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. The protein may interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins.

• NCBI Gene ID:

  3646

• Swiss Prot:

  P60228

• Accession Number:

  NP_001559

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EIF3E.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Positive Control:

  Cat. No. 1211 - HepG2 Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store EIF3E antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  52 kDa

• Applications Notes:

  EIF3E antibody can be used for detection of EIF3E by ELISA at 1:62500. EIF3E antibody can be used for detection of EIF3E by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  EIF3E

• NCBI Official Name:

  Eukaryotic translation initiation factor 3, subunit E

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Buchsbaum, S., (2007) Oncogene 26 (35), 5132-5144.

• Other Product Names:

  EIF3E, EIF3-P48, EIF3S6, INT6, eIF3-p46

• Tested Applications:

  ELISA, WB

• Protein ID:

  4503521

• Physical Properties:

  Liquid