REC8 Antibody

• Background:

  REC8 is required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II.This gene encodes a member of the kleisin family of SMC (structural maintenance of chromosome) protein partners. The protein localizes to the axial elements of chromosomes during meiosis in both oocytes and spermatocytes. In the mouse, the homologous protein is a key component of the meiotic cohesion complex, which regulates sister chromatid cohesion and recombination between homologous chromosomes. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene.

• NCBI Gene ID:

  9985

• Swiss Prot:

  O95072

• Accession Number:

  NP_005123

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human REC8.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cell Cycle

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Positive Control:

  721_B Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store REC8 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  62 kDa

• Applications Notes:

  REC8 antibody can be used for detection of REC8 by ELISA at 1:1562500. REC8 antibody can be used for detection of REC8 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  REC8

• NCBI Official Name:

  REC8 meiotic recombination protein

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Xu, H., (2005) Dev. Cell 8 (6), 949-961.

• Other Product Names:

  REC8, HR21spB, MGC950, REC8L1, Rec8p

• Tested Applications:

  ELISA, WB

• Protein ID:

  115430229

• Physical Properties:

  Liquid