SPTLC1 Antibody

• Background:

  Serine palmitoyltransferase, which consists of two different subunits, is the key enzyme in sphingolipid biosynthesis. It converts L-serine and palmitoyl-CoA to 3-oxosphinganine with pyridoxal 5'-phosphate as a cofactor. SPTLC1 is the long chain base subunit 1 of serine palmitoyltransferase. Mutations in SPTLC1 gene were identified in patients with hereditary sensory neuropathy type 1. Serine palmitoyltransferase, which consists of two different subunits, is the key enzyme in sphingolipid biosynthesis. It converts L-serine and palmitoyl-CoA to 3-oxosphinganine with pyridoxal 5'-phosphate as a cofactor. The product of this gene is the long chain base subunit 1 of serine palmitoyltransferase. Mutations in this gene were identified in patients with hereditary sensory neuropathy type 1. Alternatively spliced variants encoding different isoforms have been identified.

• NCBI Gene ID:

  10558

• Swiss Prot:

  O15269

• Accession Number:

  NP_006406

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SPTLC1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Membrane

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Positive Control:

  Cat. No. XBL-10123 - Fetal Brain Tissue Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store SPTLC1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  53 kDa

• Applications Notes:

  SPTLC1 antibody can be used for detection of SPTLC1 by ELISA at 1:62500. SPTLC1 antibody can be used for detection of SPTLC1 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  SPTLC1

• NCBI Official Name:

  Serine palmitoyltransferase, long chain base subunit 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Bi, H., (2007) Neuropathology 27 (5), 429-433.

• Other Product Names:

  SPTLC1, HSAN, HSAN1, HSN1, LBC1, LCB1, MGC14645, SPT1, SPTI

• Tested Applications:

  ELISA, WB

• Protein ID:

  5454084

• Physical Properties:

  Liquid