SPT1 Antibody

• Background:

  SPT1 Antibody: Serine palmitoyltransferase, which consists of two different subunits, is the key enzyme in sphingolipid biosynthesis. It converts L-serine and palmitoyl-CoA to 3-oxosphinganine with pyridoxal 5'-phosphate as a cofactor. SPT1 is the long chain base subunit 1 of mammalian serine palmitoyltransferase. SPT1 is not catalytically active but is necessary for the stabilization of the SPT2 subunit and anchoring the holoenzyme to the cytosolic face of the endoplasmic reticulum. Missense mutations in this gene have been identified in patients with hereditary sensory neuropathy type 1 (HSAN1) . These mutations induce a shift in the substrate specificity of the holoenzyme, leading to the formation and accumulation of two neurotoxic sphingolipids.

• NCBI Gene ID:

  10558

• Swiss Prot:

  O15269

• Accession Number:

  NP_6406

• Host:

  Rabbit

• Reactivity:

  Human

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Homeostasis

• Purification:

  SPT1 Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1201 - HeLa Cell Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Mouse: (88%), Bovine: (76%)

• Buffer:

  SPT1 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  SPT1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Specificity:

  At least two isoforms of SPT1 are known to exist; this antibody will detect only isoform a. SPT1 antibody is predicted to not cross-react with SPT2.

• Symbol:

  SPTLC1

• Positive Control 2:

  Cat. No. 1203 - A549 Cell Lysate

• Positive Control 3:

  Cat. No. 10-101 - Human Lung Tissue Slide

• NCBI Official Name:

  Serine palmitoyltransferase, long chain base subunit 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Batheja AD, Uhlinger DJ, Carton JM, et al. Characterization of serine palmitoyltransferase in normal human tissues. J. Histochem. & Cytochem. 2003; 51:687-96.

• Background Reference 02:

  Hanada K, Hara T, Nishijima N, et al. A mammalian homolog of the yeast LCB1 encodes a component of serine palmitoyltransferase, the enzyme catalyzing the first step in sphingolipid synthesis. J. biol. Chem. 1997; 272:32108-14.

• Background Reference 03:

  Yasuda S, Nishijima N, and Hanada K. Localization, topology, and function of the LCB1 subunit of serine palmitoyltransferase in mammalian cells. J. Biol. Chem. 2003; 278:4176-83.

• Background Reference 04:

  Penno A, Reilly MM, Houlden H, et al. Hereditary sensory neuropathy type 1 is caused by the accumulation of two neurotoxic sphingolipids. J. Biol. Chem. 2010; 285:11178-87.

• Other Product Names:

  SPT1 Antibody: HSN1, LBC1, LCB1, SPT1, SPTI, HSAN1, Serine palmitoyltransferase 1, Long chain base biosynthesis protein 1, LCB 1

• Tested Applications:

  ELISA, WB, IHC-P

• Protein ID:

  5454084

• Physical Properties:

  Liquid