HSD11B1 Antibody

• Background:

  HSD11B1 is a microsomal enzyme that catalyzes the conversion of the stress hormone cortisol to the inactive metabolite cortisone. In addition, HSD11B1 can catalyze the reverse reaction, the conversion of cortisone to cortisol. Too much cortisol can lead to central obesity, and a particular variation in this gene has been associated with obesity and insulin resistance in children.The protein encoded by this gene is a microsomal enzyme that catalyzes the conversion of the stress hormone cortisol to the inactive metabolite cortisone. In addition, the encoded protein can catalyze the reverse reaction, the conversion of cortisone to cortisol. Too much cortisol can lead to central obesity, and a particular variation in this gene has been associated with obesity and insulin resistance in children. Two transcript variants encoding the same protein have been found for this gene.

• NCBI Gene ID:

  3290

• Swiss Prot:

  P28845

• Accession Number:

  NP_005516

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HSD11B1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Membrane, Cancer, Signal Transduction

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Positive Control:

  Cat. No. XBL-10409 - Fetal Liver Tissue Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store HSD11B1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  32 kDa

• Applications Notes:

  HSD11B1 antibody can be used for detection of HSD11B1 by ELISA at 1:312500. HSD11B1 antibody can be used for detection of HSD11B1 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  HSD11B1

• NCBI Official Name:

  Hydroxysteroid (11-beta) dehydrogenase 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Paulsen, S.K., (2008) Obesity (Silver Spring) 16 (4), 731-735.

• Other Product Names:

  HSD11B1, 11-DH, 11-beta-HSD1, HDL, HSD11, HSD11B, HSD11L, MGC13539, CORTRD2, SDR26C1

• Tested Applications:

  ELISA, WB

• Protein ID:

  5031765

• Physical Properties:

  Liquid