ACLY Antibody

• Background:

  ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene.

• NCBI Gene ID:

  47

• Swiss Prot:

  P53396

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant fusion protein containing a sequence corresponding to amino acids 752-1101 of human ACLY (NP_001087.2) .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Signal Transduction

• Purification:

  Affinity purification

• Positive Control:

  HepG2

• Concentration:

  Batch dependent

• Buffer:

  PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at -20˚ C. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  Observed: 120kDa

• Fragment:

  IgG

• Applications Notes:

  WB: 1:200 - 1:2000

• Symbol:

  ACLY

• Positive Control 2:

  HeLa

• Positive Control 3:

  Raji

• Positive Control 4:

  NIH/3T3

• Positive Control 5:

  Mouse brain

• Positive Control 6:

  Mouse heart

• NCBI Official Name:

  ATP citrate lyase

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  ACL, ATPCL, CLATP, ATP-citrate synthase, ATP-citrate (pro-S-) -lyase, citrate cleavage enzyme

• Tested Applications:

  WB

• Physical Properties:

  Liquid