MDM2 Antibody

• Background:

  This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells.

• NCBI Gene ID:

  4193

• Swiss Prot:

  Q00987

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant fusion protein containing a sequence corresponding to amino acids 200-430 of human MDM2 (NP_002383.2) .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Cell Cycle, Immunology, Neuroscience, Signal Transduction

• Purification:

  Affinity purification

• Positive Control:

  HT-29

• Concentration:

  Batch dependent

• Buffer:

  PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at -20˚ C. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  Observed: 52kDa

• Fragment:

  IgG

• Applications Notes:

  WB: 1:500 - 1:2000

• Symbol:

  MDM2

• Positive Control 2:

  SKOV3

• Positive Control 3:

  MCF7

• Positive Control 4:

  Mouse thymus

• Positive Control 5:

  Mouse lung

• NCBI Official Name:

  MDM2 oncogene, E3 ubiquitin protein ligase

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  MDM2, Mdm2, transformed 3T3 cell double minute 2, p53 binding protein (mouse), HGNC:6973, MGC71221, hdm2, mouse double minute 2 homolog, mouse double minute 2, human homolog of, p53-binding protein, p53-binding protein MDM2, ubiquitin-protein ligase E3 Mdm2, HDMX, ACTFS

• Tested Applications:

  WB

• Physical Properties:

  Liquid