RAP1GAP Antibody

• Background:

  This gene encodes a type of GTPase-activating-protein (GAP) that down-regulates the activity of the ras-related RAP1 protein. RAP1 acts as a molecular switch by cycling between an inactive GDP-bound form and an active GTP-bound form. The product of this gene, RAP1GAP, promotes the hydrolysis of bound GTP and hence returns RAP1 to the inactive state whereas other proteins, guanine nucleotide exchange factors (GEFs), act as RAP1 activators by facilitating the conversion of RAP1 from the GDP- to the GTP-bound form. In general, ras subfamily proteins, such as RAP1, play key roles in receptor-linked signaling pathways that control cell growth and differentiation. RAP1 plays a role in diverse processes such as cell proliferation, adhesion, differentiation, and embryogenesis. Alternative splicing results in multiple transcript variants encoding distinct proteins.

• NCBI Gene ID:

  5909

• Swiss Prot:

  P47736

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Recombinant fusion protein containing a sequence corresponding to amino acids 508-727 of human RAP1GAP (NP_001139130.1) .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Signal Transduction

• Purification:

  Affinity purification

• Positive Control:

  U-87MG

• Concentration:

  Batch dependent

• Buffer:

  PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at -20˚ C. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  Observed: 90kDa

• Fragment:

  IgG

• Applications Notes:

  WB: 1:1000 - 1:2000

• Symbol:

  RAP1GAP

• Positive Control 2:

  293T

• Positive Control 3:

  SGC-7901

• Positive Control 4:

  LO2

• NCBI Official Name:

  RAP1 GTPase activating protein

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  RAP1GA11, RAP1GAPII, RAPGAP, RAP1GAP, rap1 GTPase-activating protein 1

• Tested Applications:

  WB

• Physical Properties:

  Liquid