RAP1A Antibody

• Background:

  The product of this gene belongs to the family of RAS-related proteins. These proteins share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between RAP proteins and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. The product of this gene counteracts the mitogenic function of RAS because it can interact with RAS GAPs and RAF in a competitive manner. Two transcript variants encoding the same protein have been identified for this gene.

• NCBI Gene ID:

  5906

• Swiss Prot:

  P62834

• Accession Number:

  P62834

• Host:

  Rabbit

• Reactivity:

  Mouse

• Immunogen:

  This RAP1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 142-170 amino acids from the C-terminal region of human RAP1A.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine, Rat, Chicken, Zebrafish, Human, Monkey, Xenopus

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  21 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  RAP1A

• NCBI Official Name:

  Ras-related protein Rap-1A

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Liu, C., et al. Mol. Cell. Biol. 30 (16) :3956-3969 (2010)

• Background Reference 02:

  Kelly, P., et al. J. Biol. Chem. 285 (21) :15777-15785 (2010)

• Background Reference 03:

  Ahmed, S.M., et al. J. Biol. Chem. 285 (9) :6538-6551 (2010)

• Background Reference 04:

  Hsu, Y.H., et al. PLoS Genet. (6), E1000977 (2010) :

• Other Product Names:

  Ras-related protein Rap-1A, C21KG, G-22K, GTP-binding protein smg p21A, Ras-related protein Krev-1, RAP1A, KREV1

• Tested Applications:

  WB

• Protein ID:

  51338607

• Physical Properties:

  Liquid