DFFB Antibody

• Background:

  Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of some of these variants has not been determined.

• NCBI Gene ID:

  1677

• Swiss Prot:

  O76075

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  Recombinant fusion protein containing a sequence corresponding to amino acids 109-338 of human DFFB (NP_004393.1) .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Apoptosis, Cell Cycle

• Purification:

  Affinity purification

• Positive Control:

  A-549

• Concentration:

  Batch dependent

• Buffer:

  PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at -20˚ C. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  Observed: 39kDa

• Fragment:

  IgG

• Symbol:

  DFFB

• Positive Control 2:

  K-562

• Positive Control 3:

  SW620

• Positive Control 4:

  Mouse spleen

• Positive Control 5:

  Mouse thymus

• NCBI Official Name:

  DNA fragmentation factor subunit beta

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  CAD, CPAN, DFF-4DFF2, DFF4DNA fragmentation factor subunit beta, DNA fragmentation factor 40 kDa subunit, DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase), caspase-activated DNase, caspase-activated deoxyribonuclease, caspase-activated nuclease

• Tested Applications:

  WB, IHC

• Physical Properties:

  Liquid