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DFFA Antibody, KO Validated

CAT: 0223-13-578Size: 100 µLDry Ice: NoHazardous: No
CAT#:0223-13-578Size:100 µL
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Background
Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
NCBI Gene ID
1676
Swiss Prot
O00273
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-331 of human DFFA (NP_004392.1) .
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Validation
Antibody is Knockout validated.
Field of Research
Apoptosis, Cell Cycle
Purification
Affinity purification
Positive Control
Jurkat
Concentration
Batch dependent
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store at -20˚ C. Avoid freeze / thaw cycles.
Calculated Molecular Weight
Observed: 35kDa, 45kDa
Fragment
IgG
Symbol
DFFA
Positive Control 2
H460
Positive Control 3
HepG2
Positive Control 4
Mouse brain
Positive Control 5
Rat brain
NCBI Official Name
DNA fragmentation factor, 45kDa, alpha polypeptide
NCBI Organism
Homo sapiens
Other Product Names
DFF45 Antibody: DFF1, ICAD, DFF-45, DFF1, DFF45, H13, DNA fragmentation factor subunit alpha, DNA fragmentation factor 45 kDa subunit
Tested Applications
WB, IF
Physical Properties
Liquid

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