DFFA Antibody, KO Validated

• Background:

  Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

• NCBI Gene ID:

  1676

• Swiss Prot:

  O00273

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant fusion protein containing a sequence corresponding to amino acids 1-331 of human DFFA (NP_004392.1) .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Validation:

  Antibody is Knockout validated.

• Field of Research:

  Apoptosis, Cell Cycle

• Purification:

  Affinity purification

• Positive Control:

  Jurkat

• Concentration:

  Batch dependent

• Buffer:

  PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at -20˚ C. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  Observed: 35kDa, 45kDa

• Fragment:

  IgG

• Symbol:

  DFFA

• Positive Control 2:

  H460

• Positive Control 3:

  HepG2

• Positive Control 4:

  Mouse brain

• Positive Control 5:

  Rat brain

• NCBI Official Name:

  DNA fragmentation factor, 45kDa, alpha polypeptide

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  DFF45 Antibody: DFF1, ICAD, DFF-45, DFF1, DFF45, H13, DNA fragmentation factor subunit alpha, DNA fragmentation factor 45 kDa subunit

• Tested Applications:

  WB, IF

• Physical Properties:

  Liquid