SPIB Antibody

• Background:

  SPIB is a member of the ETS transcription factor family that influences lymphoid development and activity and binds the consensus DNA site GGA (A/T) through a unique winged helix-turn-helix motif known as the ETS domain (1) . SPIB is found in hematopoietic cells such as B cells and plasmacytoid dendritic cells (DC) (2,3) . It promotes the development of plasmacytoid dendritic cells (pDCs) or natural interferon (IFN) -producing cells. SPIB may be required for B-cell receptor (BCR) signaling, which is necessary for normal B-cell development and antigenic stimulation (3,4) .

• NCBI Gene ID:

  6689

• Swiss Prot:

  Q01892

• Accession Number:

  NP_003112

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Stem Cell, Immunology

• Purification:

  SPIB antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1312 - Human Prostate Tissue Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Pig: (88%)

• Buffer:

  SPIB antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  SPIB antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

• Fragment:

  IgG

• Specificity:

  SPIB antibody is human, mouse and rat reactive. At least four isoforms of SPIB are known to exist; this antibody will detect all but isoform 3.

• Symbol:

  SPIB

• NCBI Official Name:

  Spi-B transcription factor (Spi-1/PU.1 related)

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Ray D, Bosselut R, Ghysdael J, et al. Characterization of Spi-B, a transcription factor related to the putative oncoprotein Spi-1/PU.1. Mol. Cell Biol. 1992; 12:4297-304.

• Background Reference 02:

  Nagy M, Chapuis B, and Matthes T. Expression of transcription factors Pu.1, Spi-B, Blimp-1, BSAP and oct-2 in normal human plasma cells and in multiple myeloma cells. Br. J. Haematol. 2002; 116:429-35.

• Background Reference 03:

  Schotte R, Rissoan MC, Bendriss-Vermare N, et al. The transcription factor Spi-B is expressed in plasmacytoid DC precursors and inhibits T-, B-, and NK-cell development. Blood 2003; 101:1015-23.

• Background Reference 04:

  Schotte R, Nagasawa M, Weijer K, et al. The ETS transcription factor Spi-B is required for human plasmacytoid dendritic cell development. J. Exp. Med. 2004; 200:1503-9.

• Other Product Names:

  Spi-B transcription factor, SPI-B

• Tested Applications:

  ELISA, WB, IHC-P, IF

• Protein ID:

  61888836

• Physical Properties:

  Liquid