JAK2 Antibody

• Background:

  JAK2 is a member of the JAK family of kinases that also include JAK1, JAK3, and TYK2 (1,2) . JAK kinases are activated following tyrosine phosphorylation of cytokine receptors after ligand binding. JAK2 activation promotes the recruitment and phosphorylation of STAT3 and STAT5. These transcription factors then translocate to the nucleus where they bind specific DNA promoter sequences resulting in the transcription of genes that regulate cell proliferation, differentiation, and apoptosis (3) . Mice with a disrupted JAK2 gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis (4) .

• NCBI Gene ID:

  3717

• Swiss Prot:

  O60674

• Accession Number:

  NP_004963

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Stem Cell, Apoptosis

• Purification:

  JAK2 antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1212 - 3T3 Cell Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Pig: (100%), Chicken: (82%)

• Buffer:

  JAK2 antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  JAK2 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

• Fragment:

  IgG

• Specificity:

  JAK2 antibody is human, mouse and rat reactive. JAK2 antibody is predicted to not cross-react with other members of the JAK family of proteins.

• Symbol:

  JAK2

• NCBI Official Name:

  Janus kinase 2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Wilks AF, Harpur AG, Kurban RR, et al. Two novel protein-tyrosine kinases, each with a second phosphotransferase-related catalytic domain, define a new class of protein kinase. Mol. Cell Biol. 1991; 11:2057-66.

• Background Reference 02:

  Quintas-Cardama A and Verstovsek S. Molecular pathways: JAK/STAT pathway: mutations, inhibitors, and resistance. Clin. Can. Res. 2013; 19:1933-40.

• Background Reference 03:

  Levine RL, Pardanani A, Tefferi A, et al. Role of JAK2 in the pathogenesis and therapy of myeloproliferative disorders. Nat. Rev. Cancer 2007; 7:673-83.

• Background Reference 04:

  Neubauer H, Cumano A, Muller M, et al. Jak2 deficiency defines an essential developmental checkpoint in definitive hematopoiesis. Cell 1998; 93:397-409.

• Other Product Names:

  Janus kinase 2, JTK10, THCYT3

• Tested Applications:

  ELISA, WB, IHC-P

• Protein ID:

  4826776

• Physical Properties:

  Liquid