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Vinculin Antibody

CAT: 0223-7489-01Size: 0.02 mgDry Ice: NoHazardous: No
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CAT#:0223-7489-01Size:0.02 mg
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Background
Vinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development (1) . Three structural vinculin domains include an amino-terminal head, a short flexible proline-rich region and a carboxy-terminal tail (2) . Expression of vinculin were shown to be affected by the level of actin expression (2,3) . Vinculin deficiencies are associated with a decrease in cell adhesion and an increase in cell motility, suggesting a possible role in metastatic growth (4) . Defects in VCL are the cause of cardiomyopathy dilated type 1W (CMD1W) (5) .
NCBI Gene ID
7414
Swiss Prot
P18206
Accession Number
NP_003364
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
7489
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Validation
Independent Antibody Validation in Cell lines (Figure 2) shows similar Vinculin expression profile in human, mouse and rat cell lines detected by three independent anti-Vinculin antibodies that recognize different epitopes, 7489 against C-terminus domain, 7807 against N-terminus domain and 7809 against central domain. Vinculin proteins are detected in the most tested cell lines at different expression levels by the three independent antibodies. Independent Antibody Validation in Cell lines7489 78077809
Field of Research
Homeostasis
Purification
Vinculin Antibody is affinity chromatography purified via peptide column.
Positive Control
Cat. No. 1216 - PC-3 Cell Lysate
Concentration
1 mg/mL
Homology
Predicted species reactivity based on immunogen sequence: Pig: (100%), Chicken: (100%)
Buffer
Vinculin Antibody is supplied in PBS containing 0.02% sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Vinculin antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.
Fragment
IgG
Specificity
Vinculin antibody is human, mouse and rat specific. At least three isoforms of Vinculin are known to exist.
Isoform
Human Vinculin has 3 isoforms, including isoform 2 (1134aa, 124kD), isoform 1 (1066aa, 117kD) and isoform 3 (222aa, 25kD) . Mouse Vinculin has one isoform (1066aa, 117kD) and Rat Vinculin also has one isoform (1066aa, 117kD) . 7489 can detect human, mouse and rat.
Symbol
VCL
Positive Control 2
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 3
Cat. No. 1203 - A549 Cell Lysate
Positive Control 4
Cat. No. 1282 - 3T3/NIH Cell Lysate
NCBI Official Name
Vinculin
NCBI Organism
Homo sapiens
Background Reference 01
Burridge et al. Annu. Rev. Cell Biol.1988; 4:487-525.
Background Reference 02
Gilmore et al. J. Cell Sci. 1992; 103:719-31.
Background Reference 03
Deakin et al. PLoS One 2012; 7:e37990.
Background Reference 04
Goldmann et al. Cell Biol. Int. 2013; Feb 1.
Citation 01
26873631
Other Product Names
Vinculin Antibody : MV, MVCL, CMD1W, CMH15, HEL114, Vinculin, Metavinculin, MV
Tested Applications
ELISA, WB, IHC-P, IF
Protein ID
4507877
Physical Properties
Liquid

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