PRR5L Antibody

• Background:

  PRR5L Antibody: Proline-rich protein 5-like (PRR5L), also known as Protor-2, is structurally similar to PRR5 and also binds to the mTOR complex 2 (mTORC2) . PRR5L is not required for mTORC2 integrity or kinase activity, but dissociates from mTORC2 upon knockdown of tuberous sclerosis complex 1 (TSC1) and TSC2. Knockdown of PRR5L reduced apoptosis in mTORC2 deficient cells as well as cells where mTOR was active, suggesting that mTORC2-dissociated PRR5L may promote apoptosis, and thus play a role in the balance between cell growth and cell death.

• NCBI Gene ID:

  79899

• Swiss Prot:

  Q6MZQ0

• Accession Number:

  NP_001153639

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Apoptosis

• Purification:

  PRR5L Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1282 - 3T3 (NIH) Cell Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine: (100%), Rat: (88%)

• Buffer:

  PRR5L Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  PRR5L antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Specificity:

  At least three isoforms of PRR5L are known to exist; this antibody will recognize only isoforms a and b. PRR5L antibody will not cross-react with PPR5.

• Symbol:

  PRR5L

• Positive Control 2:

  Cat. No. 17-010 - HEK293 Cell Slide

• Positive Control 3:

  Cat. No. 17-201 - 3T3/BALB Cell Slide

• NCBI Official Name:

  Proline rich 5 like

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Thedieck K, Polak P, Kim ML, et al. 2007. PRAS40 and PRR5-like protein are new mTOR interactors that regulate apoptosis. PLoS One 2007; 2:e1217.

• Background Reference 02:

  Pearce LR, Huang X, Boudeau J, et al. Identification of Protor as a novel Rictor-binding component of mTOR complex-2. Biochem. J. 2007; 405:513-22.

• Other Product Names:

  PRR5L Antibody: PROTOR2, PROTOR2, Proline-rich protein 5-like, Protein observed with Rictor-2, Protor-2

• Tested Applications:

  ELISA, WB, ICC, IF

• Protein ID:

  237757351

• Physical Properties:

  Liquid