Mouse Prr5 Antibody

• Background:

  Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. PRR5 plays an important role in regulation of PDGFRB expression and in modulation of platelet-derived growth factor signaling. May act as a tumor suppressor in breast cancer (By similarity) .

• NCBI Gene ID:

  109270

• Swiss Prot:

  Q812A5

• Accession Number:

  Q812A5

• Host:

  Rabbit

• Reactivity:

  Mouse

• Immunogen:

  This Mouse Prr5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 277-305 amino acids from the C-terminal region of mouse Prr5.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  43 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  Prr5

• NCBI Official Name:

  Proline-rich protein 5

• NCBI Organism:

  Mus musculus

• Background Reference 01:

  Johnstone, C.N., et al. Genomics 85 (3) :338-351 (2005)

• Background Reference 02:

  Shan, Z., et al. Gene 303, 55-61 (2003) :

• Other Product Names:

  Proline-rich protein 5, Protein observed with Rictor-1, Protor-1, Prr5 {ECO:0000250|UniProtKB:P85299}, Protor1

• Tested Applications:

  WB

• Physical Properties:

  Liquid