VPS53 Antibody

• Background:

  VPS53 Antibody: The sorting of acid hydrolases to lysosomes rely on mannose 6-phosphate receptors that cycle between the trans-Golgi network (TGN) and endosomes. The maintenance of this cycle requires the function of the mammalian Golgi-associated retrograde protein (GARP) complex which is composed of three subunits: VPS52, VPS53, and VPS54. Depletion of any of these three proteins, such as by RNAi, impairs the retrograde transport of multiple TGN proteins. VPS53 was identified as an HIV dependency factor (HDF) and plays a role in viral entry to the cell, suggesting that VPS53 may be an important drug target in HIV treatment. At least five isoforms of VPS53 are known to exist.

• NCBI Gene ID:

  55275

• Swiss Prot:

  Q5VIR6

• Accession Number:

  EAW90658

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Homeostasis

• Purification:

  VPS53 Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1210 - HEK293 Cell Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Chicken: (78%)

• Buffer:

  VPS53 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  VPS53 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Symbol:

  VPS53

• NCBI Official Name:

  Vacuolar protein sorting 53 homolog (S. cerevisiae)

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Pfeffer SR. Targeting of proteins to the lysosome. Curr. Top. Microbiol. Immunol.1991; 170:43-65.

• Background Reference 02:

  Liewen H, Meinhold-Heerlein I, Oliveira V, et al. Characterization of the human GARP (Golgi associated retrograde protein) complex. Exp. Cell Res.2005; 306:22-34.

• Background Reference 03:

  Perez-Victoria FJ, Mardones GA, and Bonifacino JS. Requirement of the human GARP complex for mannose 6-phosphate-receptor-dependent sorting of cathepsin D to lysosomes. Mol. Biol. Cell2008; 19:2350-62.

• Background Reference 04:

  Brass AL, Dykxhoorn DM, Benita Y, et al. Identification of host proteins required for HIV infection through a functional genomic screen. Science2008; 319:921-6.

• Other Product Names:

  VPS53 Antibody: HCCS1, hVps53L, pp13624, Vacuolar protein sorting-associated protein 53 homolog

• Tested Applications:

  ELISA, WB

• Protein ID:

  119611064

• Physical Properties:

  Liquid