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MANF Antibody

CAT: 0223-4347-01Size: 0.02 mgDry Ice: NoHazardous: No
CAT#:0223-4347-01Size:0.02 mg
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
MANF Antibody: MANF, also known as ARMET, was initially identified as a protein containing an arginine-rich region that was highly mutated in a variety of tumors. More recently it was identified as a mesencephalic astrocyte-derived neurotrophic factor with selectivity for dopaminergic neurons, similar to glial cell line-derived neurotrophic factor (GDNF) and CDNF. In rat brain slices, MANF enhanced nigral gamma-aminobutyric acid release. Like GDNF and CDNF, MANF has selective neuroprotective activity for dopaminergic neurons suggesting that it may be indicated for the treatment of Parkinson's disease. Expression of MANF has also been shown to be induced during ER stress, suggesting that it may play a role in protein quality control during ER stress.
NCBI Gene ID
7873
Swiss Prot
P55145
Accession Number
P55145
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Validation
KO Validation (Figure 1) : Anti-MANF antibodies (4347) specificity was further verified by MANF specific knockout. MANF signal was not detected in MANF knockout HEK293T cells as compared to that in control wild type cells. KO Validation4347) Recombinant Protein Test (Figure 3) : Anti-MANF antibodies (4347) detected human MANF recombinant protein at different concentrations. Recombinant Protein Test4347 Localization Validation (Figure 8) : MANF detected by anti-MANF antibodies is colocalized with HuC/D (a general neural marker) . MANF is localized in mature neuronal cells. Localization Validation KD Validation (Figure 9) : Anti-MANF antibody (4347) specificity was further verified by MANF specific knockdown. MANF signal, when injected with a combination of two antisense splice-blocking morpholino oligonucleotides (MOsp), was disrupted in comparison with either wild type or when injected with control MO . KD Validation4347) Induced Expression Validation (Figure 10) : MANF expression detected by anit-MANF antibodies was up-regulated by VPA treatment. Induced Expression Validation
Field of Research
Neuroscience
Purification
MANF Antibody is Protein A purified.
Positive Control
Cat. No. 1403 - Mouse Brain Tissue Lysate
Concentration
1 mg/mL
Homology
Predicted species reactivity based on immunogen sequence: Bovine: (92%)
Buffer
MANF Antibody is supplied in PBS containing 0.02% sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
MANF antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Fragment
IgG
Specificity
This antibody does not cross-react with CDNF.
Isoform
Human MANF has one isoform (182aa, 21kD) . Mouse MANF has one isoform (179aa, 20kD) and Rat MANF also has one isoform (179aa, 20kD) . 4347 can detect human, mouse and rat.
Symbol
MANF
Positive Control 2
Cat. No. 10-301 - Human Brain Tissue Slide
Positive Control 3
Cat. No. 1224 – Daudi Cell Lysate
Positive Control 4
Cat. No. 1282 – 3T3/NIH Lysate
NCBI Official Name
Mesencephalic astrocyte derived neurotrophic factor
NCBI Organism
Homo sapiens
Background Reference 01
Shridhar et al. Oncogene1996; 12:1931-9.
Background Reference 02
Shridhar et al. Cancer Res.1996; 56:5576-8.
Background Reference 03
Petrova et al. J. Mol. Neurosci.2003; 20:173-88.
Background Reference 04
Lindholm et al. Nature 2007; 448:73-7.
Citation 01
22898306
Citation 02
24460582
Citation 03
22243807
Other Product Names
MANF Antibody: ARP, ARMET, ARP, Mesencephalic astrocyte-derived neurotrophic factor, Arginine-rich protein
Tested Applications
ELISA, WB, IHC-P, IF
Protein ID
23503040
Physical Properties
Liquid

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