Products for Research Use Only
RegisterLogin

PD-L2 Antibody

CAT: 0223-4063-01Size: 0.02 mgDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0223-4063-01Size:0.02 mg
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Background
PD-L2 Antibody: Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by co gnate peptides bound to MHC molecules on antigen-presenting cells (APC) . T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PD-L1 and PD-L2, both of which are thought act as a negative regulator of T cell activation. However, it has been suggested that PD-L2 can act to stimulate an immunogenic response through and alternative receptor from PD-1.
NCBI Gene ID
80380
Swiss Prot
Q9BQ51
Accession Number
NP_079515
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
4063
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Validation
Independent Antibody Validation in Cell lines (Figure 2) shows similar PD-L2 expression profile in human and mouse cell lines detected by two independent anti-PD-L2 antibodies that recognize different epitopes, 4063 against central domain and the competitor antibody against N-terminus domain. PD-L2 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines4063 competitor antibody KO Validation (Figure 3) : Anti-PD-L2 antibodies (4063) specificity was further verified by PD-L2 specific knockout. PD-L2 signal was not detected in PD-L2 knockout HeLa cells as compared to that in control wild type cells. KO Validation4063) Recombinant Protein Test (Figure 4) : Anti-PD-L2 antibodies (4063) detected human PD-L2 recombinant protein at different concentrations. Recombinant Protein Test4063 Regulated expression validation (Figure 11) : PD-L2 expression detected by anit-PD-L2 antibodies (4063) was up-regulated by anti-PD1 antibody treatment, which was reduced by Next A alone or combination treatment (anti-PD1 antibody + NextA) . Regulated expression validation4063
Field of Research
Apoptosis
Purification
PD-L2 Antibody is affinity chromatography purified via peptide column.
Positive Control
Cat. No. 1207 - Raji Cell Lysate
Concentration
1 mg/mL
Homology
Predicted species reactivity based on immunogen sequence: rat (94%) .
Buffer
PD-L2 Antibody is supplied in PBS containing 0.02% sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
PD-L2 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Fragment
IgG
Isoform
Human PD-L2 has 3 isoforms, including isoform 1 (273aa, 31kD), isoform 2 (183aa, 21kD) and isoform 3 (182aa, 21kD) . Mouse PD-L2 has one isoform (247aa, 28kD) and Rat PD-L2 also has one isoform (268aa, 30kD) . 4063 can detect human isoform and mouse isoform.
Symbol
PDCD1LG2
Positive Control 2
Cat. No. 1403 - Mouse Brain Tissue Lysate
Positive Control 3
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 4
Cat. No. 1224 - Daudi Cell Lysate
Positive Control 5
Cat. No. 1282 - 3T3/NIH Cell Lysate
NCBI Official Name
Programmed cell death 1 ligand 2
NCBI Organism
Homo sapiens
Background Reference 01
Holling et al. Hum. Immunol. 2004; 65:282-90.
Background Reference 02
Ishida et al. EMBO J. 1992; 11:3887-95.
Background Reference 03
LaGier and Pober. Hum. Immunol. 2006; 67:568-78.
Background Reference 04
Zhang et al. Proc. Natl. Acad. Sci. USA 2006; 103:11695-700.
Citation 01
25672395
Citation 02
30992475
Other Product Names
PD-L2 Antibody: B7DC, Btdc, PDL2, CD273, PD-L2, PDCD1L2, bA574F11.2, B7DC, Programmed cell death 1 ligand 2, Butyrophilin B7-DC, PD-1 ligand 2
Tested Applications
ELISA, WB, IHC-P, IF
Protein ID
190014605
Physical Properties
Liquid

Related Products

CatalogName
4063PPD-L2 Peptide
97-022PD-L2 Recombinant Protein
97-021PD-L2 Recombinant Protein
91-721PD-L2 Recombinant Protein
92-497PD-L2 Recombinant Protein
96-938PD-L2 Recombinant Protein
90-425CD273 [PD-L2] Recombinant Protein
90-427CD273 [PD-L2] Recombinant Protein
90-428CD273 [PD-L2] Recombinant Protein
90-426CD273 [PD-L2] non-lytic Recombinant Protein

Alternative Products

CatalogName
OM636840-01PD-L2 Antibody
B2015512Antibody to PD-L2
GTR18173484PD L2 Antibody
516765PD-L2

Popular Products