PD-L2 Antibody

• Background:

  PD-L2 Antibody: Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by co gnate peptides bound to MHC molecules on antigen-presenting cells (APC) . T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PD-L1 and PD-L2, both of which are thought act as a negative regulator of T cell activation. However, it has been suggested that PD-L2 can act to stimulate an immunogenic response through and alternative receptor from PD-1.

• NCBI Gene ID:

  80380

• Swiss Prot:

  Q9BQ51

• Accession Number:

  NP_079515

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  4063

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Validation:

  Independent Antibody Validation in Cell lines (Figure 2) shows similar PD-L2 expression profile in human and mouse cell lines detected by two independent anti-PD-L2 antibodies that recognize different epitopes, 4063 against central domain and the competitor antibody against N-terminus domain. PD-L2 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines4063 competitor antibody KO Validation (Figure 3) : Anti-PD-L2 antibodies (4063) specificity was further verified by PD-L2 specific knockout. PD-L2 signal was not detected in PD-L2 knockout HeLa cells as compared to that in control wild type cells. KO Validation4063) Recombinant Protein Test (Figure 4) : Anti-PD-L2 antibodies (4063) detected human PD-L2 recombinant protein at different concentrations. Recombinant Protein Test4063 Regulated expression validation (Figure 11) : PD-L2 expression detected by anit-PD-L2 antibodies (4063) was up-regulated by anti-PD1 antibody treatment, which was reduced by Next A alone or combination treatment (anti-PD1 antibody + NextA) . Regulated expression validation4063

• Field of Research:

  Apoptosis

• Purification:

  PD-L2 Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1207 - Raji Cell Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: rat (94%) .

• Buffer:

  PD-L2 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  PD-L2 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Isoform:

  Human PD-L2 has 3 isoforms, including isoform 1 (273aa, 31kD), isoform 2 (183aa, 21kD) and isoform 3 (182aa, 21kD) . Mouse PD-L2 has one isoform (247aa, 28kD) and Rat PD-L2 also has one isoform (268aa, 30kD) . 4063 can detect human isoform and mouse isoform.

• Symbol:

  PDCD1LG2

• Positive Control 2:

  Cat. No. 1403 - Mouse Brain Tissue Lysate

• Positive Control 3:

  Cat. No. 1201 - HeLa Cell Lysate

• Positive Control 4:

  Cat. No. 1224 - Daudi Cell Lysate

• Positive Control 5:

  Cat. No. 1282 - 3T3/NIH Cell Lysate

• NCBI Official Name:

  Programmed cell death 1 ligand 2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Holling et al. Hum. Immunol. 2004; 65:282-90.

• Background Reference 02:

  Ishida et al. EMBO J. 1992; 11:3887-95.

• Background Reference 03:

  LaGier and Pober. Hum. Immunol. 2006; 67:568-78.

• Background Reference 04:

  Zhang et al. Proc. Natl. Acad. Sci. USA 2006; 103:11695-700.

• Citation 01:

  25672395

• Citation 02:

  30992475

• Other Product Names:

  PD-L2 Antibody: B7DC, Btdc, PDL2, CD273, PD-L2, PDCD1L2, bA574F11.2, B7DC, Programmed cell death 1 ligand 2, Butyrophilin B7-DC, PD-1 ligand 2

• Tested Applications:

  ELISA, WB, IHC-P, IF

• Protein ID:

  190014605

• Physical Properties:

  Liquid