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IL-27 Antibody

CAT: 0223-3797-01Size: 0.02 mgDry Ice: NoHazardous: No
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CAT#:0223-3797-01Size:0.02 mg
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Background
IL-27 Antibody: Like interleukin-23 (IL-23), IL-27 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. It exists as a heterodimer composed of the p40-related protein EBI3 and an IL-12 p35-related protein termed p28. IL-27 is produced after activation by antigen-presenting cells and induces proliferation of naïve but not memory CD4+ T-cells. It acts by binding to its receptor WSX-1 and gp130 which results in the activation of a Jak/STAT signaling cascade, suggesting the IL-27 is involved in the regulation of immune processes. It has been suggested that IL-27 can also be used as a therapeutic agent against cancer as it can also induce tumor-specific anti-tumor activity mediated through CD8+ T-cells, IFN-gamma, and T-bet.
NCBI Gene ID
246778
Swiss Prot
Q8NEV9
Accession Number
NP_663634
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
3797
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Validation
Independent Antibody Validation in Cell lines (Figure 2) shows similar IL-27 expression profile in human and mouse cell lines detected by two independent anti-IL-27 antibodies that recognize different epitopes, 3797 against N-terminus domain and 3799 against central domain. IL-27 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines3797 3799 Recombinant Protein Test (Figure 3) shows that anti-IL-27 antibody (3797) detects human recombinant IL-17 (IL-17A + IL-17B) protein that contains the amino acids of 3797 antigenic peptide. Recombinant Protein Test
Field of Research
Immunology, Cancer
Purification
IL-27 Antibody is affinity chromatography purified via peptide column.
Positive Control
Cat. No. 1288 - A20 Cell Lysate
Concentration
1 mg/mL
Homology
Predicted species reactivity based on immunogen sequence: Rat: (63%)
Buffer
IL-27 Antibody is supplied in PBS containing 0.02% sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
IL-27 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Fragment
IgG
Isoform
Human IL-27 has one isoform (243aa, 28kD) . Mouse IL-27 has one isoform (234aa, 27kD) and rat IL-27 also has one isoform (234aa, 27kD) . 3797 can detect human, mouse and rat IL-27.
Symbol
IL27
Positive Control 2
Cat. No. 17-207 - EL4 Cell Slide
Positive Control 3
Cat. No. 1288 - A20 Cell Lysate
Positive Control 4
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 5
Cat. No. 1205 - Jurkat Cell Lysate
Positive Control 6
Cat. No. 1220 - SK-N-SH Cell Lysate
NCBI Official Name
Interleukin 27
NCBI Organism
Homo sapiens
Background Reference 01
Hunter et al. Nat. Rev. Immunol. 2005; 5:521-31.
Background Reference 02
Pfanz et al. J. Immunol. 2004; 172:2225-31.
Background Reference 03
Hisada et al. Cancer Res. 2004; 64:1152-6.
Other Product Names
IL-27 Antibody: p28, IL30, IL-27, IL27A, IL-27A, IL27p28, Interleukin-27 subunit alpha, p28, IL-27 subunit alpha
Tested Applications
ELISA, WB, ICC
Protein ID
28416913
Physical Properties
Liquid

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