Complement C4A (C4A) (NM_007293) Human Recombinant Protein

• Background:

  This gene encodes the acidic form of complement factor 4, part of the classical activation pathway. The protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains prior to secretion. The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. The alpha chain is cleaved to release C4 anaphylatoxin, an antimicrobial peptide and a mediator of local inflammation. Deficiency of this protein is associated with systemic lupus erythematosus and type I diabetes mellitus. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. Varying haplotypes of this gene cluster exist, such that individuals may have 1, 2, or 3 copies of this gene. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014]

• Synonyms:

  C4; C4A2; C4A3; C4A4; C4A6; C4AD; C4S; CO4; CPAMD2; RG

• Gene ID:

  720

• UniProt:

  P0C0L4

• Accession Number mRNA:

  NM_007293

• Chromosomal Location:

  6p21.33

• Expression System:

  HEK293

• Tag:

  C-HIS

• Related Pathways:

  Complement and coagulation cascades, Systemic lupus erythematosus

• Field of Research:

  Complement and coagulation cascades, Systemic lupus erythematosus

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  PBS, pH7.4, 10% glycerol. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

• Molecular Weight:

  191.8 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  C4A

• Species:

  Human

• Protein ID:

  NP_009224

• Overview:

  Purified recombinant protein of Human complement component 4A (Rodgers blood group) (C4A), Lys20-End, with C-terminal His tag, secretory expressed in HEK293 cells, 50ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/720

• Uniprot URL:

  https://www.uniprot.org/uniprot/P0C0L4

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_007293

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_009224