Complement component 3 Protein, Human, Recombinant (His Tag)

CAT: 0882-PH51936M2-01Size: 100 µgDry Ice: NoHazardous: No
CAT#:0882-PH51936M2-01Size:100 µg
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Overview
A DNA sequence encoding the human complement C3 (NP_000055.2) (Met1-Asn1663) was expressed with a polyhistidine tag at the C-terminus.
Synonyms
AHUS5 Protein, Human; ARMD9 Protein, Human; ASP Protein, Human; C3a Protein, Human; C3b Protein, Human; CPAMD1 Protein, Human; HEL-S-62p Protein, Human
Gene Name
Complement component 3
Expression System
HEK293 Cells
Tag
C-His
Field of Research
Serine Proteases and Regulators
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Purity
> 90 % as determined by SDS-PAGE.
Activity
At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.
Form
Lyophilized
Buffer
Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Molecular Weight
The recombinant human complement C3 consists of 1652 amino acids and predicts a molecular mass of 186.43 kDa.
Storage Conditions
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
C3
Species
Human
Protein ID
NP_000055.2
AA Sequence
Met1-Asn1663
Protein ID Link
https://www.ncbi.nlm.nih.gov/protein/NP_000055.2

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