4-1BBL/TNFSF9 Protein, Cynomolgus, Recombinant (hFc Tag), Biotinylated

• Background:

  4-1BB ligand is the high affinity ligand of 4-1BB, also known as CD137L or TNFSF9. It is shown to be a type II surface glycoprotein belonging to the TNF superfamily. Expression of 4-1BBL is restricted to APCs, such as dendritic cells, macrophages, and activated B cells. Members of the TNF-TNF receptor superfamily have been shown to play critical roles in regulating cellular activation, differentiation and apoptosis. Several studies have reported that 4-1BBL/4-1BB interaction provided a co-stimulatory signal to T cells, and increased T cell proliferation and cytokines production. In addition, 4-1BBL is involved in cancers, infectious diseases and autoimmune diseases.

• Gene Name:

  Tumor necrosis factor (ligand) superfamily, member 9

• Expression System:

  HEK293 Cells

• Tag:

  N-hFc

• Field of Research:

  Cancer Drug Targets

• Endotoxin:

  < 1.0 EU per μg protein as determined by the LAL method.

• Purity:

  > 95 % as determined by SDS-PAGE.

• Activity:

  At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Biological Target proteins, Immunotherapy proteins, Immune Checkpoint Proteins, Receptor Proteins, Biotinylated proteins, Cancer proteins, CD Molecule proteins

• Molecular Weight:

  The recombinant cynomolgus TNFSF9 consists of 445 amino acids and predicts a molecular mass of 47.8 kDa.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  TNFSF9

• Species:

  Cynomolgus

• Protein ID:

  XP_015296398.1

• Overview:

  A DNA sequence encoding the cynomolgus TNFSF9 (XP_015296398.1) (Arg68-Glu251) was expressed with the Fc region of human IgG1 at the N-terminus. The purified protein was biotinylated in vitro.

• AA Sequence:

  Arg68-Glu251

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/XP_015296398.1