4-1BBL/TNFSF9 Protein, Rat, Recombinant (hFc Tag)

• Background:

  4-1BB ligand is the high affinity ligand of 4-1BB, also known as CD137L or TNFSF9. It is shown to be a type II surface glycoprotein belonging to the TNF superfamily. Expression of 4-1BBL is restricted to APCs, such as dendritic cells, macrophages, and activated B cells. Members of the TNF-TNF receptor superfamily have been shown to play critical roles in regulating cellular activation, differentiation and apoptosis. Several studies have reported that 4-1BBL/4-1BB interaction provided a co-stimulatory signal to T cells, and increased T cell proliferation and cytokines production. In addition, 4-1BBL is involved in cancers, infectious diseases and autoimmune diseases.

• Gene Name:

  Tumor necrosis factor (ligand) superfamily, member 9

• UniProt:

  Q80WE6

• Expression System:

  HEK293 Cells

• Tag:

  N-hFc

• Field of Research:

  Cancer Drug Targets

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 96 % as determined by SDS-PAGE.

• Activity:

  Measured by its binding ability in a functional ELISA.Immobilized canine TNFRSF9-His (Cat:PO55260M2) at 10 μg/ml (100 μl/well) can bind rat Fc-TNFSF9, The ECof rat Fc-TNFSF9 is 0.26-0.62 μg/ml.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  TNF Superfamily proteins, CD Molecule proteins, Cancer proteins, Receptor Proteins, Immunotherapy proteins, Cytokine Proteins, Immune Checkpoint Proteins, Biological Target proteins

• Molecular Weight:

  The recombinant rat TNFSF9 comprises 463 amino acids and predicts a molecular mass of 50.9 kDa. The apparent molecular mass of the recombinant protein is approximately 61 kDa in SDS-PAGE under reducing conditions due to glycosylation.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  TNFSF9

• Species:

  Rat

• Overview:

  A DNA sequence encoding the rat TNFSF9 (Q80WE6) (Pro106-Gly308) was expressed, fused with Fc region of human IgG1 at the N-terminus.

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q80WE6

• AA Sequence:

  Pro106-Gly308