GITR Protein, Human, Recombinant (His & AVI Tag), Biotinylated

• Background:

  GITR, also known as TNFRSF18 (CD357), belongs to the tumor necrosis factor receptor (TNF-R) superfamily. It is the receptor for TNFSF18. GITR plays a key role in dominant immunological self-tolerance maintained by CD25 (+) CD4 (+) regulatory T cells. GITR may be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. GITR and its ligand are important costimulatory molecules in the pathogenesis of autoimmune diseases. It also mediates NF-kappa-B activation via the TRAF2/NIK pathway.

• Synonyms:

  AITR Protein, Human; CD357 Protein, Human; GITR Protein, Human; GITR-D Protein, Human

• Gene Name:

  Tumor necrosis factor receptor superfamily, member 18

• Expression System:

  HEK293 Cells

• Tag:

  C-His & AVI

• Field of Research:

  Cancer Drug Targets

• Endotoxin:

  < 1.0 EU per μg protein as determined by the LAL method.

• Purity:

  > 95 % as determined by SDS-PAGE.

• Activity:

  Immobilized Human GITRL mFc (Cat:PH52279M4) at 2 μg/mL (100 μL/well) can bind Human GITR, Biotinylated (Cat:PH51666M2), the ECis 15-90 ng/mL.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Molecular Weight:

  The recombinant human TNFRSF18 consists of 162 amino acids and predicts a molecular mass of 17.79 kDa. It migrates as an approximately 29.13 kDa band in SDS-PAGE under reducing conditions.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  TNFRSF18

• Species:

  Human

• Protein ID:

  NP_683700.1

• Overview:

  A DNA sequence encoding the Human TNFRSF18 (NP_683700.1) (Met1-Glu161) was expressed with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.

• AA Sequence:

  Met1-Glu161

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/NP_683700.1