GITR Protein, Cynomolgus, Recombinant (His Tag)

• Background:

  GITR, also known as TNFRSF18 (CD357), belongs to the tumor necrosis factor receptor (TNF-R) superfamily. It is the receptor for TNFSF18. GITR plays a key role in dominant immunological self-tolerance maintained by CD25 (+) CD4 (+) regulatory T cells. GITR may be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. GITR and its ligand are important costimulatory molecules in the pathogenesis of autoimmune diseases. It also mediates NF-kappa-B activation via the TRAF2/NIK pathway.

• Gene Name:

  Tumor necrosis factor receptor superfamily, member 18

• Expression System:

  HEK293 Cells

• Tag:

  C-His

• Field of Research:

  Cancer Drug Targets

• Endotoxin:

  < 1.0 EU per μg protein as determined by the LAL method.

• Purity:

  > 95 % as determined by SDS-PAGE.

• Activity:

  At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Immune Checkpoint Proteins, Biological Target proteins, Cytokine Proteins, TNF Superfamily proteins, CD Molecule proteins, Receptor Proteins, Cancer proteins, Immunotherapy proteins

• Molecular Weight:

  The recombinant Cynomolgus TNFRSF18 consists of 147 amino acids and predicts a molecular mass of 16.1 kDa.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  TNFRSF18

• Species:

  Cynomolgus

• Protein ID:

  XP_005545180.1

• Overview:

  A DNA sequence encoding the Cynomolgus TNFRSF18 (XP_005545180.1) (Met1-Glu155) was expressed with a polyhistidine tag at the C-terminus.

• AA Sequence:

  Met1-Glu155

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/XP_005545180.1