Rat BMP8A (Bone Morphogenetic Protein 8A) Microsample ELISA Kit

CAT: 0855-ELK7096MS-01Size: 48 TestsDry Ice: NoHazardous: No
CAT#:0855-ELK7096MS-01Size:48 Tests
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Product Name Alternative
BMP8-A
Reactivity
Rat
Field of Research
Developmental science; Bone metabolism
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat BMP8A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat BMP8A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat BMP8A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat BMP8A in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
20 ng/mL
Sample Type
Serum, plasma and other biological fluids.
Detection Range
0.32-20 ng/mL
Sensitivity
0.107 ng/mL

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