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Rat BMP4 (Bone Morphogenetic Protein 4) Microsample ELISA Kit

CAT: 0855-ELK8635MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK8635MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
BMP2B; BMP2-B; BMP2B1; ZYME; DVR4; Bone morphogenetic protein 2B
UniProt
Q06826
Reactivity
Rat
Field of Research
Developmental science; Bone metabolism
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat BMP4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat BMP4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat BMP4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat BMP4 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
1000 pg/mL
Sample Type
Serum, plasma and other biological fluids
Detection Range
15.63-1000 pg/mL
Sensitivity
6 pg/mL

UniProtKB · Q06826

Bone morphogenetic protein 4

BMP4_RAT · Rattus norvegicus

View on UniProt ↗
Primary accession
Q06826
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Bmp4
Protein existence
2: Evidence at transcript level
Organism
Rattus norvegicus (Rat)
Taxonomy ID
10116
Alternative names
BMP-4
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

Biological process

ChondrogenesisDifferentiationOsteogenesis

PTM

Cleavage on pair of basic residuesDisulfide bondGlycoproteinPhosphoprotein

Molecular function

CytokineDevelopmental proteinGrowth factor

Cellular component

Extracellular matrixSecreted

Technical term

Reference proteome

Domain

Signal

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