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Human BMP8B (Bone Morphogenetic Protein 8B) Microsample ELISA Kit

CAT: 0855-ELK5121MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK5121MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
BMP8-B; OP2; Osteogenic Protein 2
UniProt
P34820
Reactivity
Human
Field of Research
Developmental science; Bone metabolism
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BMP8B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BMP8B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BMP8B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BMP8B in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
1000 pg/mL
Sample Type
Serum, plasma, tissue homogenates and other biological fluids.
Detection Range
15.63-1000 pg/mL
Sensitivity
7 pg/mL

UniProtKB · P34820

Bone morphogenetic protein 8B

BMP8B_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P34820
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
BMP8B
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
BMP-8, BMP-8B
EC number
—
Processing
Precursor
Secondary accessions
E7EMY8, Q32NE5, Q53ZM7, Q9NUF0
Protein keywords

Coding sequence diversity

Alternative splicing

Biological process

ChondrogenesisDifferentiationOsteogenesis

Molecular function

CytokineDevelopmental proteinGrowth factor

PTM

Disulfide bondGlycoprotein

Technical term

Proteomics identificationReference proteome

Cellular component

Secreted

Domain

Signal

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