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Human BMP1 (Bone Morphogenetic Protein 1) Microsample ELISA Kit

CAT: 0013-GTR18873115-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18873115-01Size:48 Tests
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Description
Human BMP1 (Bone Morphogenetic Protein 1) Microsample ELISA Kit
Product Name Alternative
PCOLC; PCP; TLD; mTld; Procollagen C Proteinase; Mammalian tolloid protein
UniProt
P13497
Reactivity
Human
Field of Research
Stem Cell & Developmental Biology; Musculoskeletal & Connective Tissue Research
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Sensitivity
6 pg/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BMP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BMP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BMP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BMP1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
15.63-1000 pg/mL

UniProtKB · P13497

Bone morphogenetic protein 1

BMP1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P13497
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
BMP1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
BMP-1
EC number
3.4.24.19
Processing
Precursor
Secondary accessions
A8K6F5, B2RN46, D3DSR0, Q13292, Q13872, Q14874, Q99421, Q99422, Q99423, Q9UL38
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Ligand

CalciumMetal-bindingZinc

Biological process

ChondrogenesisDifferentiationOsteogenesis

PTM

Cleavage on pair of basic residuesDisulfide bondGlycoproteinMethylationZymogen

Molecular function

CytokineDevelopmental proteinGrowth factorHydrolaseMetalloproteaseProtease

Disease

Disease variantOsteogenesis imperfecta

Domain

EGF-like domainRepeatSignal

Cellular component

Extracellular matrixGolgi apparatusSecreted

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