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Human BMP4 (Bone Morphogenetic Protein 4) Microsample ELISA Kit

CAT: 0855-ELK7500MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK7500MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
BMP2B; BMP2-B; BMP2B1; ZYME; DVR4; Bone morphogenetic protein 2B
UniProt
P12644
Reactivity
Human
Field of Research
Developmental science; Bone metabolism
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BMP4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BMP4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BMP4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BMP4 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
2000 pg/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
31.25-2000 pg/mL
Sensitivity
12.6 pg/mL

UniProtKB · P12644

Bone morphogenetic protein 4

BMP4_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P12644
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
BMP4
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
BMP-4
EC number
—
Processing
Precursor
Secondary accessions
Q9UM80
Protein keywords

Biological process

ChondrogenesisDifferentiationOsteogenesis

PTM

Cleavage on pair of basic residuesDisulfide bondGlycoproteinPhosphoprotein

Molecular function

CytokineDevelopmental proteinGrowth factor

Disease

Disease variantMicrophthalmia

Cellular component

Extracellular matrixSecreted

Technical term

Proteomics identificationReference proteome

Domain

Signal

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