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Human BMP3 (Bone Morphogenetic Protein 3) Microsample ELISA Kit

CAT: 0855-ELK3297MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK3297MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
BMP3A; BMP-3A; Osteogenic
UniProt
P12645
Reactivity
Human
Field of Research
Developmental science; Bone metabolism
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BMP3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BMP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BMP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BMP3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
2000 pg/mL
Sample Type
Serum, plasma, tissue homogenates and other biological fluids.
Detection Range
31.25-2000 pg/mL
Sensitivity
11.6 pg/mL

UniProtKB · P12645

Bone morphogenetic protein 3

BMP3_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P12645
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
BMP3
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
BMP-3
EC number
—
Processing
Precursor
Secondary accessions
Q4VAS5
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Biological process

ChondrogenesisDifferentiationOsteogenesis

PTM

Cleavage on pair of basic residuesDisulfide bondGlycoprotein

Molecular function

CytokineDevelopmental proteinGrowth factor

Cellular component

Secreted

Domain

Signal

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