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Human C8g (Complement Component 8g) Microsample ELISA Kit

CAT: 0855-ELK2720MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK2720MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
C8C; C8G; CO8G_HUMAN; Complement component C8 gamma chain; MGC142186
UniProt
P07360
Reactivity
Human
Field of Research
Metabolic pathway; Immune molecule; Hematology
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C8g. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C8g. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C8g, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human C8g in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Serum, plasma and other biological fluids.
Detection Range
0.16-10 ng/mL
Sensitivity
0.068 ng/mL

UniProtKB · P07360

Complement component C8 gamma chain

CO8G_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P07360
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
C8G
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
Q14CT8, Q14CU0, Q5SQ07
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Biological process

Complement alternate pathwayComplement pathwayCytolysisImmunityInnate immunity

PTM

Disulfide bondPyrrolidone carboxylic acid

Cellular component

MembraneMembrane attack complexSecretedTarget cell membraneTarget membrane

Ligand

Retinol-binding

Domain

Signal

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