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Rat C4a (Complement Component 4a) Microsample ELISA Kit

CAT: 0855-ELK11116MS-01Size: 48 TestsDry Ice: NoHazardous: No
CAT#:0855-ELK11116MS-01Size:48 Tests
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Product Name Alternative
C4a anaphylatoxin
Reactivity
Rat
Field of Research
Immune molecule; Endocrinology
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat C4a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat C4a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat C4a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat C4a in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 μg/mL
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.16-10 μg/mL
Sensitivity
0.05 μg/mL

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