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Human C3a (Complement Component 3a) Microsample ELISA Kit

CAT: 0855-ELK10298MS-01Size: 48 TestsDry Ice: NoHazardous: No
CAT#:0855-ELK10298MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
Complement Component 3a
UniProt
P01024
Reactivity
Human
Field of Research
Infection immunity; Immune molecule; Autoimmunity
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C3a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C3a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C3a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human C3a in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
500 ng/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
7.81-500 ng/mL
Sensitivity
2.04 ng/mL

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