Rat C4 (Complement Component 4) Microsample ELISA Kit

CAT: 0855-ELK11086MS-01Size: 48 TestsDry Ice: NoHazardous: No
CAT#:0855-ELK11086MS-01Size:48 Tests
Selected
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product image 1
1 / 1
Product Name Alternative
C4BP-a; Complement Component 4 Binding Protein, Alpha
UniProt
P08649
Reactivity
Rat
Field of Research
Signal transduction; Infection immunity; Hematology
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat C4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat C4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat C4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat C4 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
500 μg/mL
Sample Type
Serum, plasma and other biological fluids
Detection Range
7.82-500 μg/mL
Sensitivity
2.22 μg/mL

Popular Products