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Human MUC4 (Mucin 4) Microsample ELISA Kit

CAT: 0855-ELK1740MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK1740MS-01Size:48 Tests
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Product Name Alternative
ASGP; Mucin 4 Cell Surface Associated; Mucin 4, Tracheobronchial; Testis mucin; Ascites sialoglycoprotein; Pancreatic adenocarcinoma mucin
UniProt
Q99102
Reactivity
Human
Field of Research
Tumor immunity; Infection immunity; Pulmonology
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MUC4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MUC4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MUC4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MUC4 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
20 ng/mL
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
0.32-20 ng/mL
Sensitivity
0.127 ng/mL

UniProtKB · Q99102

Mucin-4

MUC4_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q99102
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
MUC4
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
MUC-4
EC number
—
Processing
Precursor
Secondary accessions
O95938, Q9GZM2, Q9GZV6, Q9H481, Q9H482, Q9H483, Q9H484, Q9H485, Q9H486, Q9H487, Q9H4D6, Q9H4D8, Q9NPJ0, Q9NY09, Q9NY75, Q9NY76, Q9NY77, Q9NY78, Q9NY79, Q9NY80, Q9NY81
Protein keywords

Coding sequence diversity

Alternative splicing

Biological process

Cell adhesion

Cellular component

Cell membraneMembraneSecreted

PTM

Disulfide bondGlycoprotein

Domain

EGF-like domainRepeatSignalTransmembraneTransmembrane helix

Technical term

Proteomics identificationReference proteome

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