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Human MUC2 (Mucin 2) Microsample ELISA Kit

CAT: 0855-ELK2010MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK2010MS-01Size:48 Tests
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Product Name Alternative
MLP; SMUC; Oligomeric Mucus/Gel-Forming; Mucin 2, Intestinal/Tracheal
UniProt
Q02817
Reactivity
Human
Field of Research
Tumor immunity; Infection immunity; Gastroenterology
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MUC2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MUC2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MUC2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MUC2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
100 ng/mL
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
1.57-100 ng/mL
Sensitivity
0.55 ng/mL

UniProtKB · Q02817

Mucin-2

MUC2_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q02817
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
MUC2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
MUC-2
EC number
—
Processing
Precursor
Secondary accessions
Q14878
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

Autocatalytic cleavageDisulfide bondGlycoproteinPhosphoprotein

Ligand

CalciumCopper

Domain

RepeatSignal

Cellular component

Secreted

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