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Rat MUC2 (Mucin 2) Microsample ELISA Kit

CAT: 0855-ELK3950MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK3950MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
MLP; SMUC; Oligomeric Mucus/Gel-Forming; Mucin 2, Intestinal/Tracheal
UniProt
Q62635
Reactivity
Rat
Field of Research
Tumor immunity; Infection immunity; Gastroenterology
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MUC2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MUC2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MUC2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MUC2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
20 ng/mL
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
0.32-20 ng/mL
Sensitivity
0.113 ng/mL

UniProtKB · Q62635

Mucin-2

MUC2_RAT · Rattus norvegicus

View on UniProt ↗
Primary accession
Q62635
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Muc2
Protein existence
1: Evidence at protein level
Organism
Rattus norvegicus (Rat)
Taxonomy ID
10116
Alternative names
MUC-2
EC number
—
Processing
Fragment,Precursor
Secondary accessions
—
Protein keywords

PTM

Autocatalytic cleavageDisulfide bondGlycoprotein

Ligand

CalciumCopperMetal-binding

Technical term

Direct protein sequencingReference proteome

Domain

RepeatSignal

Cellular component

Secreted

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