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Mouse MUC4 (Mucin 4) Microsample ELISA Kit

CAT: 0855-ELK3337MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK3337MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
ASGP; Mucin 4 Cell Surface Associated; Mucin 4, Tracheobronchial; Testis mucin; Ascites sialoglycoprotein; Pancreatic adenocarcinoma mucin
UniProt
Q8JZM8
Reactivity
Mouse
Field of Research
Tumor immunity; Infection immunity; Pulmonology
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MUC4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MUC4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MUC4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MUC4 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
50 ng/mL
Sample Type
Tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Detection Range
0.79-50 ng/mL
Sensitivity
0.26 ng/mL

UniProtKB · Q8JZM8

Mucin-4

MUC4_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q8JZM8
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Muc4
Protein existence
2: Evidence at transcript level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
MUC-4
EC number
—
Processing
Precursor
Secondary accessions
Q4VAA3, Q9ERB8, Q9QXG0, Q9WV36
Protein keywords

Biological process

Cell adhesion

Cellular component

Cell membraneMembraneSecreted

PTM

Disulfide bondGlycoprotein

Domain

EGF-like domainRepeatSignalTransmembraneTransmembrane helix

Technical term

Reference proteome

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