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Mouse PLGF (Placenta Growth Factor) Microsample ELISA Kit

CAT: 0855-ELK1242MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK1242MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
PlGF2; PGF; PGFL; Placental Growth Factor-Like; Vascular Endothelial Growth Factor-Related Protein
UniProt
P49764
Reactivity
Mouse
Field of Research
Cytokine; Reproductive science
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PLGF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PLGF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PLGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PLGF in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
2000 pg/mL
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
31.25-2000 pg/mL
Sensitivity
7.96 pg/mL

UniProtKB · P49764

Placenta growth factor

PLGF_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P49764
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Pgf
Protein existence
2: Evidence at transcript level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
PlGF
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

Biological process

AngiogenesisDifferentiation

Molecular function

Developmental proteinGrowth factorMitogen

PTM

Disulfide bondGlycoprotein

Technical term

Reference proteome

Cellular component

Secreted

Domain

Signal